abstract：:Epstein-Barr virus (EBV)-transformed human B cells were seeded at densities below their capacity for autostimulatory growth and the effects of interleukin 1-alpha (IL-1-alpha), interleukin 1-beta (IL-1-beta) and B-cell growth factor (BCGF) were studied. When added separately, IL-1-alpha was able to support the growth ...

journal_title：Scandinavian journal of immunology

authors： Gallagher G,Taylor N,Willdridge J

更新日期：1987-09-01 00:00:00

abstract：:A truncated form of the class II antigen DR alpha chain of the human major histocompatibility complex was produced in bacteria. A cDNA clone encoding the intact chain was modified so that the segment encoding the signal sequence was replaced by an ATG codon and the 3' region downstream to the part corresponding to the...

journal_title：Scandinavian journal of immunology

authors： Bill P,Lind P,Rask L,Peterson PA

更新日期：1987-09-01 00:00:00

abstract：:Oral vaccination with the combined B subunit/whole cell cholera vaccine generates antitoxin memory cells that could be isolated from peripheral blood for at least 1 year after immunization. These memory cells were triggered by antigen in vitro to produce antitoxin in the presence of autologous T cells and monocytes. A...

journal_title：Scandinavian journal of immunology

authors： Lycke N,Hellström U,Holmgren J

更新日期：1987-08-01 00:00:00

abstract：:Jacalin, a D-galactose-specific lectin from jackfruit, interacts with human IgA and one or two other serum proteins. Incubation of jacalin with fresh human serum was shown to result in activation of the complement system. Therefore the mechanism of complement activation by jacalin was studied. Jacalin was extracted fr...

journal_title：Scandinavian journal of immunology

authors： Hiemstra PS,Gorter A,Stuurman ME,Van Es LA,Daha MR

更新日期：1987-08-01 00:00:00

abstract：:The Kva IgG2(k) myeloma protein showed a complete resistance to papain in the presence of cysteine at neutral pH, and a higher resistance to trypsin and alpha-chymotrypsin digestion than other IgG2 proteins. On the other hand, the Kva molecule was cleaved by pepsin at low pH to give the expected F(ab')2 fragment. When...

journal_title：Scandinavian journal of immunology

authors： Vogt RA,Michaelsen TE

更新日期：1987-07-01 00:00:00

abstract：:Chronic inflammatory synovitis is characterized by both lymphocytic infiltrates and persistent polymorph exudates. Activated polymorphs release reactive oxygen species (ROS) during inflammation, but the contribution that these make to the lymphocyte abnormalities associated with RA has been little studied. We therefor...

journal_title：Scandinavian journal of immunology

authors： Allan IM,Lunec J,Salmon M,Bacon PA

更新日期：1987-07-01 00:00:00

abstract：:Epithelial expression of class II antigens encoded by the major histocompatibility complex (MHC) has been proposed as a means by which autoimmune thyroid disease may be initiated and maintained. We studied a rat thyroid epithelial cell line (FRTL-5), which constitutively expresses class I (OX18) but not class II (OX6 ...

journal_title：Scandinavian journal of immunology

authors： Rayner DC,Lydyard PM,de Assis-Paiva HJ,Bidey S,van der Meide P,Varey AM,Cooke A

更新日期：1987-06-01 00:00:00

abstract：:Antigen 60 (A60), a member of the thermostable macromolecular antigen family (TMA) and main component of old tuberculin and purified protein derivative (PPD), has been purified from the cytoplasm of Mycobacterium bovis BCG; its structure and metabolism have already been described. In the present paper, the action of A...

journal_title：Scandinavian journal of immunology

authors： Cocito C,Baelden MC,Benoit C

更新日期：1987-06-01 00:00:00

abstract：:The role of HLA class I subunits in class II-restricted immune responses was investigated by means of a panel of monoclonal antibodies (MoAb) recognizing HLA-A,B,C heavy chain and different beta 2 microglobulin (beta 2m) epitopes. MoAb against either class I subunit strongly inhibited mixed lymphocyte cultures, genera...

journal_title：Scandinavian journal of immunology

authors： Spagnoli GC,Ausiello CM,Cassone A,Casciani CU,Bellone G,Malavasi F

更新日期：1987-06-01 00:00:00

abstract：:One hundred and twenty IgM-secreting hybridomas derived from unmanipulated 6-day-old BALB/c mice were screened for reactivity with the prototype idiotypic and anti-idiotypic monoclonal antibodies, defining three well established systems, namely TEPC 15:10/13-15, J558:CD3.2, and MOPC 460:F 6(51). Up to 25% of all IgM a...

journal_title：Scandinavian journal of immunology

authors： Araujo PM,Holmberg D,Martinez-A C,Coutinho A

更新日期：1987-05-01 00:00:00

abstract：:We have previously demonstrated that monoclonal anti-I-A/E antibodies inhibit B-cell responses to lipopolysaccharide (LPS). In the present report, the inhibitory effects were shown to be carried out directly on B cells, and to be totally independent of the LPS concentration used, thereby showing that antibodies do not...

journal_title：Scandinavian journal of immunology

authors： Forsgren S,Martinez-A C,Coutinho A

更新日期：1987-03-01 00:00:00

abstract：:Changes in the concentration and composition of serum immunoglobulin A (IgA) and deposits of IgA in tissues are well-known characteristics of alcoholic liver disease. We investigated whether these changes also accompany IgA synthesis by peripheral blood mononuclear cells (MNC), by counting immunoglobulin-producing cel...

journal_title：Scandinavian journal of immunology

authors： Van de Wiel A,Seifert WF,Van der Linden JA,Gmelig-Meyling FH,Kater L,Schuurman HJ

更新日期：1987-02-01 00:00:00

abstract：:The in vitro immune response of lymphocytes from uraemic patients was studied by comparing the in vitro kinetics of interleukin 2 (IL-2) production, the mitogen-induced proliferative response, and the expression of IL-2 receptors by T lymphocytes. The IL-2 production in 26 uraemic cell cultures decreased significantly...

journal_title：Scandinavian journal of immunology

authors： Langhoff E,Hofmann B,Odum N,Ladefoged J,Platz P,Ryder LP,Svejgaard A

更新日期：1987-01-01 00:00:00

abstract：:Intraperitoneal infection of young adult C57BL/6 males with 10(5) blood or cloned culture forms of Trypanosoma cruzi (CL strain) induced the appearance in spleen, blood, and lymph nodes of cytotoxic effector cells detectable in a lectin-dependent 51Cr-release assay. The effector cells were conventional cytotoxic T lym...

journal_title：Scandinavian journal of immunology

authors： Minoprio PM,Coutinho A,Joskowicz M,D'Imperio Lima MR,Eisen H

更新日期：1986-12-01 00:00:00

abstract：:The lymphocyte subsets in the bronchoalveolar lavage fluid (BALF) and the peripheral blood of 25 healthy volunteers were examined by analysis with a fluorescence-activated cell sorter. Comparison of the lymphocyte subsets in the BALF with those of the peripheral blood revealed much higher values for the ratios of each...

journal_title：Scandinavian journal of immunology

authors： Yamada M,Tamura N,Shirai T,Kira S

更新日期：1986-11-01 00:00:00

abstract：:Drainage of the thoracic duct resulted in a decrease in the IgA level in rat bile, but at the same time there was an increase in both the total IgM level and the specific IgM antibody activity to Escherichia coli 06 in the bile of animals immunized in the Peyer's patches with these bacteria. The increase in total IgM ...

journal_title：Scandinavian journal of immunology

authors： Dahlgren UI,Ahlstedt S,Hanson LA

更新日期：1986-11-01 00:00:00

abstract：:Guinea pig alpha 1-microglobulin (alpha 1-m) was found to exert mitogenic effects on guinea pig lymphocytes, i.e. peritoneal exudate lymphocytes (PEL) or T lymphocytes from regional or mesenteric lymph nodes (LNL). On the other hand, spleen T lymphocytes did not respond to alpha 1-m. Stimulation of LNL required the pr...

journal_title：Scandinavian journal of immunology

authors： Lögdberg L,Akerström B,Shevach EM

更新日期：1986-11-01 00:00:00

abstract：:The in vitro effect of superoxide anion and lysosomal enzyme activity on the killing of Listeria monocytogenes EGD (listeria) by peritoneal macrophages (PM) was investigated. Generation of superoxide anion by PM stimulated with phorbol myristate acetate (PMA) was significantly increased by intraperitoneal injection of...

journal_title：Scandinavian journal of immunology

authors： Hashimoto S,Nomoto K,Yokokura T

更新日期：1986-10-01 00:00:00

abstract：:The monoclonal antibodies A and B specific for the HN molecule of Sendai virus were used to induce polyclonal and monoclonal anti-idiotypic antibodies. No response was observed in allogeneic Lewis rats, low responses in syngeneic LOU rats, and high responses in allogeneic BN rats and xenogeneic Balb/c mice. The monocl...

journal_title：Scandinavian journal of immunology

authors： Fenner M,Siegmann K,Binz H

更新日期：1986-09-01 00:00:00

abstract：:When the virus dose is increased from 10(2) (low dose) to 10(4) LD50 (high dose) a fatal lymphocytic choriomeningitis virus (LCMV) infection is changed into a subclinical one, and a selective virus-specific delayed-type hypersensitivity (DTH) unresponsiveness is induced, while the cytotoxic T-cell response remains ess...

journal_title：Scandinavian journal of immunology

authors： Marker O,Thomsen AR

更新日期：1986-08-01 00:00:00

abstract：:Human peripheral blood monocyte subsets with and without Fc receptor for human IgG are known to suppress (FcR+) and enhance (FcR-) pokeweed mitogen-induced polyclonal immunoglobulin synthesis in vitro. The ability of these subsets to modulate immunoglobulin production in the presence or absence of OKT8+ T cells and un...

journal_title：Scandinavian journal of immunology

authors： Pryjma J,Flad HD,Gruber M,Ernst M

更新日期：1986-07-01 00:00:00

abstract：:Fresh human T lymphocytes from the blood of healthy individuals exhibited few motile forms, i.e. nonspherical shape and lamellar surface activity, when allowed to settle on a plastic surface. This poor motility of 'normal' blood T lymphocytes is most likely physiological, since under the same conditions more than 75% ...

journal_title：Scandinavian journal of immunology

authors： Sundqvist KG,Otteskog P,Mellstedt H

更新日期：1986-06-01 00:00:00

abstract：:Amyloid was extracted from the liver of a horse that had developed amyloidosis after being used for several years for the production of antibodies to bacterial antigens. The amyloid fibrils were shown to be of the AA type. Two AA proteins with molecular weights of 9000 and 11,000 and with identical partial N-terminal ...

journal_title：Scandinavian journal of immunology

authors： Husebekk A,Husby G,Sletten K,Marhaug G,Nordstoga K

更新日期：1986-06-01 00:00:00

abstract：:To evaluate the level of lymphocyte activation in reactive and rheumatoid arthritis, density gradient-isolated, synovial fluid mononuclear cells were stained with a panel of antisera directed at lymphocyte activation markers using an avidin-biotin-peroxidase complex (ABC) method. More specifically, we studied the expr...

journal_title：Scandinavian journal of immunology

authors： Konttinen YT,Nordström D,Bergroth V,Leirisalo-Repo M,Skrifvars B

更新日期：1986-06-01 00:00:00

abstract：:The build-up of long-term immunological memory in the gut mucosal immune system may explain long-lasting protection against new attacks of cholera in convalescents from natural disease. We have looked for gut mucosal antitoxin immunological memory and memory cells in mice after oral immunization with cholera toxin. Ou...

journal_title：Scandinavian journal of immunology

authors： Lycke N,Holmgren J

更新日期：1986-05-01 00:00:00

abstract：:We have studied the interaction of mitogenic lectins such as phytohaemagglutinin (PHA) and concanavalin A (Con A) with both surface molecules which, by the use of monoclonal antibodies, are known to trigger T-cell mitogenesis. Monoclonal antibodies recognizing the T-lymphocyte receptor for antigen (Ti) and/or its asso...

journal_title：Scandinavian journal of immunology

authors： Leca G,Boumsell L,Fabbi M,Reinherz EL,Kanellopoulos JM

更新日期：1986-05-01 00:00:00

abstract：:Affinity-purified human IgG anti-tetanus antibody was subjected to papain and then pepsin digestion, and residual fragments retaining antibody activity were re-isolated by adsorption and elution from Sepharose-tetanus toxoid columns. Both Fab' and Fv fragments isolated by gel filtration showed strong reactivity with a...

journal_title：Scandinavian journal of immunology

authors： Silvestris F,Williams RC Jr,Searles RP

更新日期：1986-04-01 00:00:00

abstract：:Three strains of mice bearing the autosomal recessive lpr gene (MRL, C57BL/6, and C3H) that had spontaneously developed a lupus-like disease were studied sequentially for functional natural killer (NK) and natural cytotoxic (NC) cell activity. Natural killing was impaired in spleen and bone marrow cells from all the l...

journal_title：Scandinavian journal of immunology

authors： Pan LZ,Dauphinée MJ,Ansar Ahmed S,Talal N

更新日期：1986-04-01 00:00:00

abstract：:Native agarose beads (diameter 5-10 micron), activators of the alternative complement pathway, are slowly phagocytosed when incubated with human monocytes cultured under serum-free conditions. Agarose beads preincubated with monocyte cultures and then transferred to new cultures are more easily phagocytosed than nativ...

journal_title：Scandinavian journal of immunology

authors： Hetland G,Eskeland T

更新日期：1986-03-01 00:00:00

abstract：:Twenty-eight children infected with Schistosoma haematobium and S. mansoni were tested for natural killer (NK) cell activity in vitro using the myeloid/erythroid cell line K562 as target. In addition, the frequency of large granular lymphocytes (LGL) and the number of HNK-1+ lymphocytes were examined in peripheral blo...

journal_title：Scandinavian journal of immunology

authors： Gastl GA,Feldmeier H,Kortmann C,Daffalla AA,Peter HH

更新日期：1986-03-01 00:00:00

abstract：:The study shows that serum from pneumococcal polysaccharide type III (SSSIII)-immunized and nonimmunized mice that has been absorbed until devoid of anti-SSSIII antibodies inhibits anti-SSSIII plaque-forming cells (PFC) but not antisheep red blood PFC in antibody plaque assay. A large fraction of the inhibitions activ...

journal_title：Scandinavian journal of immunology

authors： Pasanen VJ

更新日期：1986-03-01 00:00:00

abstract：:Cells produced in murine T-cell colonies grown in methylcellulose have been studied to evaluate their proliferation, phenotypic differentiation, and ability to give rise to cytotoxic cells. Colony-forming cells from presumably cortical or medullary thymocytes, separated by peanut agglutination, or from lymph nodes, sh...

journal_title：Scandinavian journal of immunology

authors： Röpke C,Claësson MH

更新日期：1986-02-01 00:00:00

abstract：:Immature monocytes residing in the spleens of newborn mice less than 6-7 days of age are known to suppress T-dependent and T-independent immune responses in vitro and in vivo. Suppression is mediated in part through products secreted by the newborn monocytes. In the present study, we have identified 3 monokines secret...

journal_title：Scandinavian journal of immunology

authors： Jadus MR,Peck AB

更新日期：1986-01-01 00:00:00

abstract：:Immunohistological techniques were used to identify activated T lymphocytes within the synovial membrane of patients with rheumatoid arthritis, using the monoclonal antibody (MoAb) RFT2, which identifies a 40-k dalton molecule preferentially expressed by T blasts or activated cells. Using this reagent together with a ...

journal_title：Scandinavian journal of immunology

authors： Poulter LW,Duke O,Panayi GS,Hobbs S,Raftery MJ,Janossy G

更新日期：1985-12-01 00:00:00

abstract：:Rabbit anti-beta 2 microglobulin antibodies have been described as a potent mitogen for human B lymphocytes. However, when fractionated after activation, only the T-cell enriched population is actively dividing. The induction of proliferation in purified T cells requires the presence of non-T cells. Daudi cells (which...

journal_title：Scandinavian journal of immunology

authors： Hammarström L,Smith CI

更新日期：1985-12-01 00:00:00

abstract：:With the use of the fluorescent Ca++ indicator Quin-2, we have measured changes in intracellular calcium levels in human B cells in response to anti-Ig antibodies, to Staphylococcus aureus (Staph) or to protein A. Cells of an Epstein-Barr virus-transformed mu lambda-carrying B-cell line, AZU-1, increased free cytosoli...

journal_title：Scandinavian journal of immunology

authors： Clevers HC,Bloem AC,Gmelig-Meyling F,Ballieux RE

更新日期：1985-11-01 00:00:00

abstract：:We describe the effects of treatment with topical steroids or dithranol on T and dendritic cells in the skin of patients with chronic plaque psoriasis. Resolution of lesions by both types of topical treatments was accompanied by a marked decrease in epidermal T cells. In steroid treated lesions there was also a reduct...

journal_title：Scandinavian journal of immunology

authors： Baker BS,Swain AF,Griffiths CE,Leonard JN,Fry L,Valdimarsson H

更新日期：1985-11-01 00:00:00

abstract：:Two cytokines, interferon (IFN) and interleukin 2 (IL-2), activate murine natural killer (NK) cells in vitro. Together both factors synergize considerably. Antibody against IFN eliminates the response of NK cells to IFN as well as to IL-2, whereas antibody against IL-2 blocks the effect of IL-2 but not of IFN. These f...

journal_title：Scandinavian journal of immunology

authors： Chun M,Krim M,Granelli-Piperno A,Hirst JA,Hoffmann MK

更新日期：1985-10-01 00:00:00

abstract：:The fibril in primary amyloidosis (AL) is composed of a monoclonal light chain or portions thereof. No unique primary structure has been identified that predisposes certain light chains to form amyloid fibrils. Currently, classification of amyloidosis is based on the biochemistry of the amyloid fibril. We determined t...

journal_title：Scandinavian journal of immunology

authors： Gertz MA,Skinner M,Cohen AS,Connors LH,Kyle RA

更新日期：1985-09-01 00:00:00

abstract：:Peripheral blood mononuclear cells from a donor with a high titre of anti-hepatitis B surface (HBs) antibodies were fused with a cell line that was positive for Epstein-Barr virus nuclear antigen and sensitive to hypoxanthine-aminopterine-thymidine. A cell line was established that produces a monoclonal IgG1 lambda an...

journal_title：Scandinavian journal of immunology

authors： Stricker EA,Tiebout RF,Lelie PN,Zeijlemaker WP

更新日期：1985-09-01 00:00:00